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© IRL Press Limited

A microcomputer program for the identification of tRNA genes

Giovanni Paolella * and Tommaso Russo

Istituto di Scienze Biochimiche, ll Facolta di Medicina e Chirurgia, Università degli Studi di Napoli via S. Pansini 5, 80131 Napoli, Italy

*To whom reprint requests should be sent

A microcomputer program which locates tRNA genes within long DNA sequences is described. The search is performed either by identifying tRNA-like secondary structures or by locating eukaryotic RNA polymerase III promoter consensus sequences. The program is also useful in finding inverted repeats allowing the formation of stem-loop secondary structures in tRNA. The program has been developed in BASIC and 6502 Assembler and runs on the Apple II plus and He microcomputers. The execution is quite fast; all the operations are carried out in 1–90 s, depending on the required task and on the sequence length.

Received on March 1, 1985; accepted on April 25, 1985

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