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© IRL Press Limited

The use of microcomputers for the quantitation of light intensity patterns using digitized video signals

Timothy J. Lott , Junghui Yang 1, Jianhong Ye 1 and Douglas C. Wallace 1

Department of Biology, and Pediatrics, (Division of 'Medical Genetics), Emory University Atlanta, GA 30322, USA
1Departments of Biochemistry and Pediatrics, (Division of 'Medical Genetics), Emory University Atlanta, GA 30322, USA

We have developed an inexpensive yet versatile microcomputer-based system for quantitating light intensity levels in autoradiographs. This system employs a standard video camera interfaced to an analog-to-digital convertor. A program has been written for this system which can measure intensities within a defined region of an autoradiograph, permitting an easy and accurate quantitation of spots or bands of irregular shape.

Received on June 18, 1985; accepted on September 3, 1985

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