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© Oxford University Press

fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood

Gary J. Olsen , Hideo Matsuda 1,2, Ray Hagstrom 1 and Ross Overbeek 1

Department of Microbiology, University of Illinois Urbana, IL 61801
1Mathematics and Computer Science Division, Argonne National Laboratory Argonne, IL 60439-4801, USA

We have developed a new tool, called fastDNAml, for constructing phylogenetic trees from DNA sequences. The program can be run on a wide variety of computers ranging from Unix workstations to massively parallel systems, and is available from the Ribosomal Database Project (RDP) by anonymous FTP. Our program uses a maximum likelihood approach and is based on version 3.3 of Felsenstein's dnaml program. Several enhancements, including algorithmic changes, significantly improve performance and reduce memory usage, making it feasible to construct even very large trees. Trees containing 40–100 taxa have been easily generated, and phylogenetic estimates are possible even when hundreds of sequences exist. We are currently using the tool to construct a phylogenetic tree based on 473 small subunit rRNA sequences from prokaryotes.


; accepted on December 9, 1993

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