DNA fragment size determination on agarose gel by using the application GEL
Department of Oral Biology, College of Dentistry, University of Florida Gainesville, FL 32610-0424, USA
We have developed an application to calculate double-stranded DNA fragment size on an agarose gel. This application is named GEL and is implemented for two different platform: MS-DOS and Macintosh. Both implementations allow entry of the relative migration distance of a DNA fragment of an agarose gel containing up to 20 different wells and up to 20 bands per well. Up to three different standards can be present on the gel, and the application will use the closest standard to calculate the fragment size. Both applications have a familiar user interface: the Turbo Vision interface for the MS-DOS implementation and the familiar Macintosh interface for the Macintosh implementation. In addition, the MS-DOS implementation has an extensive context on-line help.
Received on October 4, 1993; accepted on December 8, 1993
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