MC-Fit: Using Monte-Carlo methods to get accurate confidence limits on enzyme parameters
Laboratoire de Biochimie, Unité de Reacherche Associée n240 du CNRS, Ecole Polytechnique 91128 Palaiseau Cedex, France
A program is described for estimating enzymatic parameters from experimental data using Apple Macintosh computers. MC-Fit uses iterative least-square fitting and Monte-Carlo sampling to get accurate estimates of the confidence limits. This approach is more robust than the conventional covariance matrix estimation, especially in cases where experimental data is partially lacking or when the standard error on individual measurements is large. This happens quite often when analysing the properties of variant enzymes obtained by mutagenesis, as these can have severely impaired activities and reduced affinities for their substrates.
Received on August 5, 1993; accepted on January 31, 1994
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B.-W. Ying, D. Fourmy, and S. Yoshizawa Substitution of the use of radioactivity by fluorescence for biochemical studies of RNA RNA, November 1, 2007; 13(11): 2042 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Pierre, J. A. Traverso, B. Boisson, S. Domenichini, D. Bouchez, C. Giglione, and T. Meinnel N-Myristoylation Regulates the SnRK1 Pathway in Arabidopsis PLANT CELL, September 1, 2007; 19(9): 2804 - 2821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wydau, M.-L. Ferri-Fioni, S. Blanquet, and P. Plateau GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase Nucleic Acids Res., February 16, 2007; 35(3): 930 - 938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Frottin, A. Martinez, P. Peynot, S. Mitra, R. C. Holz, C. Giglione, and T. Meinnel The Proteomics of N-terminal Methionine Cleavage Mol. Cell. Proteomics, December 1, 2006; 5(12): 2336 - 2349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Yatime, E. Schmitt, S. Blanquet, and Y. Mechulam Functional Molecular Mapping of Archaeal Translation Initiation Factor 2 J. Biol. Chem., April 16, 2004; 279(16): 15984 - 15993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Serero, C. Giglione, A. Sardini, J. Martinez-Sanz, and T. Meinnel An Unusual Peptide Deformylase Features in the Human Mitochondrial N-terminal Methionine Excision Pathway J. Biol. Chem., December 26, 2003; 278(52): 52953 - 52963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Boisson, C. Giglione, and T. Meinnel Unexpected Protein Families Including Cell Defense Components Feature in the N-Myristoylome of a Higher Eukaryote J. Biol. Chem., October 31, 2003; 278(44): 43418 - 43429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brevet, J. Chen, S. Commans, C. Lazennec, S. Blanquet, and P. Plateau Anticodon Recognition in Evolution: SWITCHING tRNA SPECIFICITY OF AN AMINOACYL-tRNA SYNTHETASE BY SITE-DIRECTED PEPTIDE TRANSPLANTATION J. Biol. Chem., August 15, 2003; 278(33): 30927 - 30935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Steer and P. Schimmel Major Anticodon-binding Region Missing from an Archaebacterial tRNA Synthetase J. Biol. Chem., December 10, 1999; 274(50): 35601 - 35606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Steer and P. Schimmel Domain-domain communication in a miniature archaebacterial tRNA synthetase PNAS, November 23, 1999; 96(24): 13644 - 13649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Soutourina, P. Plateau, F. Delort, A. Peirotes, and S. Blanquet Functional Characterization of the D-Tyr-tRNATyr Deacylase from Escherichia coli J. Biol. Chem., July 2, 1999; 274(27): 19109 - 19114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Brevet, J. Chen, F. Lévque, S. Blanquet, and P. Plateau Comparison of the Enzymatic Properties of the Two Escherichia coli Lysyl-tRNA Synthetase Species J. Biol. Chem., June 16, 1995; 270(24): 14439 - 14444. [Abstract] [Full Text] [PDF]
|
|