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© Oxford University Press

TRACTOR, a program to locate subclones in a nucleotide sequence using only one sequencing reaction

J.T. Niemi and P. Mäntsälä

Department of Biochemistry and Food Chemistry, University of Turku Arcanum, Vatselankatu 2, FIN-20500, Turku, Finland

The QuickBasic program TRACTOR is used to locate clones in a known sequence using only one sequencing reaction, a method generally known as ‘T-tracking’. The program can reduce workload at the end stage of a sequencing project using the random subcloning strategy.


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