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© Oxford University Press

Accurate determination of DNA in agarose gels using the novel algorithm GelScann(1.0)

Michael L. Metzker , Kyle M. Allain 1 and Richard A. Gibbs

Department of Molecular and Human Genetics, Baylor College of Medicine Houston, TX 77030, USA

GelScann(1.0) is a user-friendly program that accurately quantitates DNA from CCD imaged agarose gels. The algorithm automatically locates lanes, locates bands within a given lane, and quantitates the intensity of each band. GelScann(1.0) uses a statistical method to discriminate DNA from local backgrornd fluorescence. The sensitivity of the LaneFinder and BandFinder can be adjusted by the user interacting with GelScann(1.0) 's option box. We demonstrate that GelScann(1.0) can accurately determine DNA concentrations for routine molecular biology applications and determine the relative intensities of PCR amplified fragments for genetic testing.

Received on August 1, 1994; accepted on October 28, 1994

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