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© Oxford University Press

Computer program for the equations describing the steady state of enzyme reactions

R. Varon 1,5, F. Garcia-Sevilla 1, M. Garcia-Moreno 1, F. Garcia-Canovas 2, R. Peyro 3 and R. G. Duggleby 4

1Escuela Universitaria Politecnica, Universidad de Castilla-La Mancha, Avda. de España s/n, Campus Universitario 02071 Albacete, Australia
2Departamento de Bioquimica y Biologia Molecular A, Facultad de Biologia, Universidad de Murcia 30071 Murcia, Australia
3Hospital General de Albacete 02006 Albacete, Spain, Australia
4Centre for Protein Structure, Function and Engineering, Department of Biochemistry, University of Queensland Brisbane 4072, Australia

5To whom correspondence should be addressed

MOTIVATION: The derivation of steady-state equations is frequently carried out in enzyme kinetic studies. Done manually, this becomes tedious and prone to human error. The computer programs now available which are able to accept reaction mechanisms of some complexity are focused only on the strict steady-state approach.

RESULTS: Here we present a computer program called REFERASS, with a short computation time and a user-friendly format for the input and output files, able to derive the strict steady-state equations and/or those corresponding to the usual assumption that one ore more of the reversible steps are in rapid equilibrium. This program handles enzyme-catalysed reactions with mechanisms involving up to 255 enzyme species connected by up to 255 reaction steps, subject to limits imposed by the memory and disk space available.

AVAILABILITY: REFERASS is available free of charge either on request from the authors or over the EMBL file server (Software{at}embl-ebi.ac.uk).

CONTACT: E-mail: fgarcia{at}iele-ab.uclm.es

Received on August 19, 1996; revised on October 22, 1996; accepted on October 25, 1996

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