Skip Navigation

This Article
Right arrow FREE Full Text (Print PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hooft, R. W.W.
Right arrow Articles by Vriend, G.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Hooft, R. W.W.
Right arrow Articles by Vriend, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press

Objectively judging the quality of a protein structure from a Ramachandran plot

Rob W.W. Hooft , Chris Sander and Gerrit Vriend

EMBL Meyerhofstraße 1. D-69117 Heidelberg, Germany

MOTIVATION: Statistical methods that compare observed and expected distributions of experimental observables provide powerful tools for the quality control of protein structures. The distribution of backbone dihedral angles (‘Ramachandran plot’) has often been used for such quality control, but without a firm statistical foundation.

RESULTS: A new and simple method is presented for judging the quality of a protein structure based on the distribution of backbone dihedral angles. Inputs to the method are 60 torsion angle distributions extracted from protein structures solved at high resolution; one for each combination of residue type and tri-state secondary structure. Output for a protein is a Ramachandran Z-score, expressing the quality of the Ramachandran plot relative to current state-of-the-art structures.

AVAILABILITY: The Ramachandran test is available as part of the free WHAT_CHECK program. Information about this program can be obtained on the WWW from http://swift.embl-heidelberg.de/whatcheck/

CONTACT: E-mail: r.hooft{at}euromail.com

Received on December 6, 1996; accepted on March 27, 1997

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
A. V. Pandey, P. Kempna, G. Hofer, P. E. Mullis, and C. E. Fluck
Modulation of Human CYP19A1 Activity by Mutant NADPH P450 Oxidoreductase
Mol. Endocrinol., October 1, 2007; 21(10): 2579 - 2595.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.