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Bioinformatics, Vol 14, 92-97, Copyright © 1998 by Oxford University Press

ARTICLES

Trace alignment and some of its applications

R Mott
Informatics Group, Sanger Centre, Cambridge, UK.

MOTIVATION: Extra useful information can be extracted from a DNA chromatogram trace, over that contained in the base-called DNA sequence. Many sequencing applications can benefit from examination of these traces. RESULTS: An algorithm, based on dynamic programming, for aligning a DNA chromatogram to a DNA sequence is described and implemented. Its applications to vector clipping, EST alignment and mutation detection are discussed.
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