Bioinformatics Vol. 17 no. 12 2001
Pages 1226-1227
© 2001 Oxford University Press
Applications Note |
MELTING, computing the melting temperature of nucleic acid duplex
Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK
Received on April 10, 2001
; revised on June 19, 2001
; accepted on July 2, 2001
Summary: MELTING computes the enthalpy and entropy of an oligonucleotide duplex helix–coil transition, and then its melting temperature. The program uses the method of nearest-neighbours. The set of thermodynamic parameters can be easily customized. The program provides several correction methods for the concentration of salt. MELTING is a free program, available at no cost and open-source. Perl scripts are provided to show how MELTING can be used to construct more ambitious programs.
Availability: MELTING is available for several platforms (http://www.pasteur.fr/recherche/unites/neubiomol/meltinghome.html) and is accessible via a www server (http://bioweb.pasteur.fr/seqanal/interfaces/melting.html).
Contact: nl223{at}cus.cam.ac.uk
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