Bioinformatics Vol. 18 no. 12 2002
Pages 1593-1599
© 2002 Oxford University Press
Analysis of high density expression microarrays with signed-rank call algorithms
Applied Research and Product Development, Affymetrix, Inc., 3380 Central Expressway, Santa Clara, CA 95051, USA
Received on November 12, 2001
; revised on March 4, 2002
; accepted on March 21, 2002
Motivation: We consider the detection of expressed genes and the comparison of them in different experiments with the high-density oligonucleotide microarrays. The results are summarized as the detection calls and comparison calls, and they should be robust against data outliers over a wide target concentration range. It is also helpful to provide parameters that can be adjusted by the user to balance specificity and sensitivity under various experimental conditions.
Results: We present rank-based algorithms for making detection and comparison calls on expression microarrays. The detection call algorithm utilizes the discrimination scores. The comparison call algorithm utilizes intensity differences. Both algorithms are based on Wilcoxon's signed-rank test. Several parameters in the algorithms can be adjusted by the user to alter levels of specificity and sensitivity. The algorithms were developed and analyzed using spiked-in genes arrayed in a Latin square format. In the call process, p-values are calculated to give a confidence level for the pertinent hypotheses. For comparison calls made between two arrays, two primary normalization factors are defined. To overcome the difficulty that constant normalization factors do not fit all probe sets, we perturb these primary normalization factors and make increasing or decreasing calls only if all resulting p-values fall within a defined critical region. Our algorithms also automatically handle scanner saturation.
Availability: These algorithms are available commercially as part of the MAS 5.0 software package.
Contact: wei-min_liu{at}affymetrix.com
* To whom correspondence should be addressed.
Current address: Mail Code CR145,
Oregon Health Sciences University, Portland, OR 79201
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