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Bioinformatics Vol. 18 no. 12 2002
Pages 1690-1691
© 2002 Oxford University Press

Applications Note

Design of endonuclease restriction sites into primers for PCR cloning

Jianghui Hou

Molecular Medicine Center, Edinburgh University, Edinburgh EH4 2XU, UK

Received on October 10, 2001 ; revised on January 4, 2002 and June 11, 2002 ; accepted on June 17, 2002

Summary: I present a software system PCRCLNG that facilitates the design of endonuclease restriction sites into the 5'-end of PCR primers. The product amplified using these primers can be directly cloned into vectors. The program estimates the annealing temperature for each primer and selects the primer pairs with comparable annealing temperature. Finally the software determines whether the PCR product can be cloned into the vector to generate in-frame gene fusion.

Availability: PCRCLNG is available for non-commercial users free of charge as a set of executables and source codes upon request from the author.

Contact: jianghui.hou{at}ed.ac.uk


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