Bioinformatics Vol. 18 no. 2 2002
Pages 321-322
© 2002 Oxford University Press
Applications Note |
Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays
Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark
Received on July 5, 2001
; revised on October 10, 2001
; accepted on October 19, 2001
Summary: PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3'end.
Availability: PROBEWIZ is accessible from http://www.cbs.dtu.dk/services/DNAarray/probewiz.html
Contact: hbjorn{at}cbs.dtu.dk; steen{at}cbs.dtu.dk
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