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Bioinformatics Vol. 18 no. 7 2002
Pages 949-952
© 2002 Oxford University Press

A bioinformatic strategy to rapidly characterize cDNA libraries

G. Charles Ostermeier 1, David J. Dix 2 and Stephen A. Krawetz 1,*

1 Departments of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, and Institute for Scientific Computing, Wayne State University, USA
2 Office of Research and Development, US Environmental Protection Agency, USA

Received on October 13, 2001 ; revised on January 18, 2002 ; accepted on February 13, 2002

Motivation: Complementary DNA libraries can define the genetic constituents of specific cells and/or tissues. Their sequencing will illuminate the transcriptome but it is a monumental task requiring considerable resources.

Results: We have employed a computational search in conjunction with a microarray-based strategy to alleviate the impediments of deriving a consensus of records that describe testis gene expression. This strategy identified 5681 unique testes-expressed genes of which 3265 were previously portrayed in the UniGene database. Interestingly, a total of 2416 novel testes-expressed genes were identified. This clearly demonstrates that microarrays can be used to rapidly discover a large number of new transcripts.

Contact: steve{at}compbio.med.wayne.edu

* To whom correspondence should be addressed at: Charlotte B. Failing Professor, 253 C.S. Mott Center, 275 East Hancock, Detroit, MI, 48201, USA.


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