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Bioinformatics Vol. 19 no. 12 2003
Pages 1587-1588
© 2003 Oxford University Press

Applications Note

ESTWeb: bioinformatics services for EST sequencing projects

Apuã C. M. Paquola , Milton Y. Nishyiama, Jr , Eduardo M. Reis , Aline M. da Silva and Sergio Verjovski-Almeida *

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, 05508-900, São Paulo, SP, Brazil

Received on September 11, 2002 ; revised on November 3, 2002 ; accepted on March 2, 2003

Summary: ESTWeb is an internet based software package designed for uniform data processing and storage for large-scale EST sequencing projects. The package provides for: (a) reception of sequencing chromatograms; (b) sequence processing such as base-calling, vector screening, comparison with public databases; (c) storage of data and analysis in a relational database, (d) generation of a graphical report of individual sequence quality; and (e) issuing of reports with statistics of productivity and redundancy. The software facilitates real-time monitoring and evaluation of EST sequence acquisition progress along an EST sequencing project.

Availability: http://bioinfo.iq.usp.br/estweb

Contact: verjo{at}iq.usp.br

Supplementary information: http://bioinfo.iq.usp.br/estweb

* To whom correspondence should be addressed.


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