Bioinformatics Vol. 19 no. 15 2003
Pages 1972-1977
© 2003 Oxford University Press
Recognizing translation initiation sites of eukaryotic genes based on the cooperatively scanning model
1 Department of Physics, Hebei University of Technology, Tianjin 300130, China and 2 Department of Physics, Tianjin University, Tianjin 300072, China
Received on December 25, 2002
; revised on March 24, 2003
; accepted on April 17, 2003
Motivation: Translation initiation sites (TISs) of genes are the key points of protein synthesis. Exact recognition of TISs in eukaryotic genes is one of the most important tasks in gene-finding algorithms. However, the task has not been satisfactorily fulfilled up to the present. Here, we propose a cooperatively scanning model for recognizing TISs and the first exons of eukaryotic genes on the basis of the structural characteristics of multi-exon genes.
Results: The model was employed to cooperatively scan the TISs and 3' splicing sites in eukaryotic genes, and the TISs and the first exons of 132 mammalian gene sequences are identified to evaluate the model. Accuracy of exactly recognizing the TISs and the first exons has been found to amount respectively to 64.4 and 51.5%. We believe that the model will be a useful tool for genome annotation and that it can be easily incorporated into other algorithms to achieve higher accuracy in recognizing TISs and the first exons.
Availability: The program is available upon request.
Contact: yhwangwz{at}263.net