Skip Navigation

This Article
Right arrow FREE Full Text (Print PDF) Freely available
Right arrow FREE Full Text (Screen PDF)
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hsieh, M.-H.
Right arrow Articles by Tzeng, C.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsieh, M.-H.
Right arrow Articles by Tzeng, C.-M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Bioinformatics Vol. 19 no. 2 2003
Pages 285-286
© 2003 Oxford University Press

Applications Note

An efficient algorithm for minimal primer set selection

Ming-Hua Hsieh , Wei-Che Hsu , Sung-Kay Chiu and Chi-Meng Tzeng *

Department of Research and Development, U-Vision Biotech Inc., 3F 132, Ln 235, Pao-Chiao Rd, Hsin-Tien City 231 Taipei, Taiwan

Received on June 12, 2002 ; revised on July 29, 2002 ; accepted on August 6, 2002

Summary: We have developed U-PRIMER, a primer design program, to compute a minimal primer set (MPS) for any given set of DNA sequences. The U-PRIMER algorithm, which uses automatic variable fixing and automatic redundant constraint elimination to tackle the binary integer programming problem associated with the MPS selection problem. The program has been tested successfully with 32 adipocyte development-related genes and 9 TB-specific genes to obtain their respective MPSs.

Availability: A free copy of U-PRIMER implemented in C++ programming language is available from http://www.u-vision-biotech.com

Contact: cmtzeng{at}u-vision-biotech.com

* To whom correspondence should be addressed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BioinformaticsHome page
M. Leber, L. Kaderali, A. Schonhuth, and R. Schrader
A fractional programming approach to efficient DNA melting temperature calculation
Bioinformatics, May 15, 2005; 21(10): 2375 - 2382.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.