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Bioinformatics Vol. 19 no. 6 2003
Pages 776-777
© 2003 Oxford University Press

Applications Note

AFLPinSilico, simulating AFLP fingerprints

Stephane Rombauts 1,*, Yves Van de Peer 1 and Pierre Rouzé 2

1 Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), K.L. Ledeganckstraat 35, Gent 9000, Belgium
2 Laboratoire associé de l’INRA (France), K.L. Ledeganckstraat 35, Gent 9000, Belgium

Received on August 22, 2002 ; revised on October 29, 2002 ; accepted on December 13, 2002

Summary: A drawback of the Amplified Fragment Length Polymorphism (AFLP) fingerprinting method is the difficulty to correlate the different fragments with their DNA sequence. The AFLPinSilico application presented here simulates AFLP experiments run on either cDNA or genomic sequences, producing virtual fingerprints that allow high throughput identification of AFLP fragments. The program also enables biologists to manage experiments through simulations done beforehand, thereby reducing the number of experiments that have to be run. AFLPinSilico is available through the www or as a stand-alone version, through a command line executable (available upon request, for any platform running PERL).

Availability: For academic use http://www.psb.rug.ac.be/bioinformatics/AFLPinSilico.html

Contact: strom{at}gengenp.rug.ac.be

* To whom correspondence should be addressed.


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