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© IRL Press Limited

An effective approach to the identification of subclones of a DNA of known nucleotide sequence

Eu.l. Golovanov * and S.A. Nedospasov 1,2

Insitute of Molecular Genetics, USSR Academy of Sciences Moscow 123182
1Institute of Molecular Biology, USSR Academy of Sciences Moscow 117984, USSR
2Swiss Institute for Experimental Cancer Research Epalinges CH-1066, Switzerland

*To whom reprint requests should besent

The problem of subclone identification for DNA fragments of a known nucleotide sequence has been considered.We suggest a strategy for rapid identification of a large number of subclones based on: (i) partial sequencing of the subclone DNA (single nucleotide track);(ii)representation of the result in the form of a numeric code showing the distribution of the chosen nucleotide along the sequence; and (iii) identification of the subclone sequence using this code in a catalogue compiled and printed for a whole DNA sequence. The same approach is applicable when the subclones are expected to have homology with known sequences.

Received on January 24, 1986; accepted on September 11, 1986

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