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Bioinformatics Advance Access originally published online on April 8, 2004
Bioinformatics 2004 20(15):2471-2472; doi:10.1093/bioinformatics/bth254
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Bioinformatics 20(15) © Oxford University Press 2004; all rights reserved.

Applications Note

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR

Owen J. Marshall

Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic., 3052, Australia

Received on December 17, 2003; revised on March 9, 2004; accepted on March 17, 2004
Advance Access Publication April 8, 2004

Summary: PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer–dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.

Availability: Open-source and freely available from http://perlprimer.sourceforge.net

Contact: owen.marshall{at}mcri.edu.au


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