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Bioinformatics Advance Access originally published online on April 8, 2004
Bioinformatics 2004 20(15):2473-2475; doi:10.1093/bioinformatics/bth259
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Bioinformatics 20(15) © Oxford University Press 2004; all rights reserved.

Applications Note

G-PRIMER: greedy algorithm for selecting minimal primer set

Jiren Wang 1,*, Kuo-Bin Li 1 and Wing-Kin Sung 2

1 BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671 and 2 Department of Computer Science, National University of Singapore, 3 Science Drive 2, Singapore 117543

Received on January 25, 2004; revised on February 27, 2004; accepted on March 9, 2004
Advance Access Publication April 8, 2004

Summary: G-PRIMER, a web-based primer design program, has been developed to compute a minimal primer set specifically annealed to all the open reading frames in a given microbial genome. This program has been successfully used in the microarray experiment for analyzing the expression of genes in the Xanthomonas campestris genome.

Availability: It is available at http://mammoth.bii.a-star.edu.sg/gprimer/. Its source code is available upon request.

Contact: jiren{at}bii.a-star.edu.sg

* To whom correspondence should be addressed.


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