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Vol. 20 no. 2 2004, pages 271-273
Bioinformatics © Oxford University Press 2004; all rights reserved.

Applications Note

ROSO: optimizing oligonucleotide probes for microarrays

Nancie Reymond 1,*, Hubert Charles 1, Laurent Duret 2, Federica Calevro 1, Guillaume Beslon 3 and Jean-Michel Fayard 1

1 Biologie Fonctionnelle, Insectes et Interactions (BF2I), UMR INRA/INSA de Lyon, 69621 Villeurbanne, France, 2 BBE, UMR CNRS, Université Claude Bernard – Lyon 1, 69622 Villeurbanne, France and 3 PRISMa, 69621 Villeurbanne, France

Received on April 1, 2003 ; revised on June 20, 2003 ; accepted on August 5, 2003

Summary: ROSO is software to design optimal oligonucleotide probe sets for microarrays. Selected probes show no significant cross-hybridization, no stable secondary structures and their Tm are chosen to minimize the Tm variability of the probe set.

Availability: The program is available on the internet. Sources are freely available, for non-profit use, on request to the authors.

Supplementary information: http://pbil.univ-lyon1.fr/roso

Contact: nancie.reymond{at}jouy.inra.fr

* To whom correspondence should be addressed.


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