Bioinformatics Advance Access originally published online on January 22, 2004
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Bioinformatics 20(4) © Oxford University Press 2004; all rights reserved.
Discovery Note |
Translational polymorphism as a potential source of plant proteins variety in Arabidopsis thaliana
1 Institute of Cytology and Genetics, Pr. Lavrentieva 10, Novosibirsk 630090, Russia and 2 Tsukuba Institute, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Japan
Received on August 22, 2003
; accepted on September 9, 2003
Advance Access Publication January 22, 2004
Motivation: According to scanning model, 40S ribosomal subunits can either initiate translation at start AUG codon in suboptimal context or miss it and initiate translation at downstream AUG(s), thereby producing several proteins. Functional significance of such a protein translational polymorphism is still unknown.
Results: We compared predicted subcellular localizations of annotated Arabidopsis thaliana proteins and their potential N-terminally truncated forms started from the nearest downstream in-frame AUG codons. It was found that localizations of full and N-truncated proteins differ in many cases: 12.2% of N-truncated proteins acquired sorting signals de novo and 5.7% changed their predicted subcellular locations (mitochodria, chloroplast or secretory pathway). It is likely that the in-frame downstream AUGs may be frequently utilized to synthesize proteins possessing new functional properties and such a translational polymorphism may serve as an important source of cellular and organelle proteomes.
Availability: Detailed description of arabidopsis mRNAs potentially producing novel functional protein forms is available by request.
Contact: Alex Kochetov, ak{at}bionet.nsc.ru
* To whom correspondence should be addressed.
3 Present address: Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Iizuka 820-8502, Japan.
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