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Bioinformatics Advance Access originally published online on August 13, 2004
Bioinformatics 2005 21(1):122-123; doi:10.1093/bioinformatics/bth459
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Bioinformatics vol. 21 issue 1 © Oxford University Press 2005; all rights reserved.

BlastAlign: a program that uses blast to align problematic nucleotide sequences

Robert Belshaw 1,* and Aris Katzourakis 2

1 Department of Biological Sciences, Imperial College Silwood Park Campus, Ascot, Berks SL5 7PY, UK
2 Department of Zoology, University of Oxford South Parks Road, Oxford OX1 3PS, UK

*To whom correspondence should be addressed.

Summary: BlastAlign uses NCBI blastn to build a multiple nucleotide alignment and is intended for use with sequences that have large indels or are otherwise difficult to align globally. The program builds a matrix representing regions of homology along the sequences, from which it selects the ‘most representative’ sequence and then extracts the blastn query-anchored multiple alignment for this sequence. The matrix is printed and allows subgroups to be identified visually and an option allows other sequences to be used as the ‘most representative’. The program contains elements of both Perl and Python and will run on UNIX (including Mac OSX) and DOS. An additional Perl program BlastAlignP uses tblastn to align nucleotide sequences to a single amino acid sequence, thus allowing an open reading frame to be maintained in the resulting multiple alignment.

Availability: It is freely available at http://www.bio.ic.ac.uk/research/belshaw/BlastAlign.tar and at http://evolve.zoo.ox.ac.uk/software/blastalign

Contact: r.belshaw{at}imperial.ac.uk

Received on April 20, 2004; accepted on July 26, 2004

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