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Bioinformatics Advance Access originally published online on August 2, 2005
Bioinformatics 2005 21(18):3665-3666; doi:10.1093/bioinformatics/bti601
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

WebACT—an online companion for the Artemis Comparison Tool

J. C. Abbott 1,*, D. M. Aanensen 2, K. Rutherford 3,{dagger}, S. Butcher 1 and B. G. Spratt 2

1Centre for Bioinformatics, Division of Molecular Biosciences, Imperial College London London SW7 2AZ, UK
2Department of Infectious Disease Epidemiology, Imperial College London London, W2 1PG, UK
3Pathogen Sequencing Unit, Sanger Institute Cambridge CB10 1SA, UK

*To whom correspondence should be addressed.

Summary: WebACT is an online resource which enables the rapid provision of simultaneous BLAST comparisons between up to five genomic sequences in a format amenable for visualization with the well-known Artemis Comparison Tool (ACT). Comparisons can be generated on-the-fly using sequences directly retrieved via EMBL database queries, or by entering or uploading user sequences. Furthermore, pre-computed comparisons are available between all publicly available, completed prokaryotic genomes and plasmids currently contained within the Genome Reviews database (372 sequences, representing 175 different species). The system is designed to minimize the volume of downloaded data and maximize performance. Genome sequences, annotation and pre-computed comparisons are stored in a relational database allowing flexible querying based on user-defined sequence regions, from whole genome to a defined region flanking a specified gene. Comparison and sequence files, whether computed online or retrieved from the database of pre-computed genome comparisons, can be viewed online using ACT and are available for download.

Availability: Freely accessible at http://www.webact.org

Contact: admin{at}webact.org

Supplementary information: User guide and worked examples are available at http://www.webact.org/WebACT/docs

Received on May 23, 2005; revised on July 27, 2005; accepted on July 27, 2005

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