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Bioinformatics Advance Access originally published online on August 9, 2006
Bioinformatics 2006 22(20):2554-2555; doi:10.1093/bioinformatics/btl434
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© 2006 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (
http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

MASQOT-GUI: spot quality assessment for the two-channel microarray platform

Max Bylesjö 1,*, Andreas Sjödin 2, Daniel Eriksson 3, Henrik Antti 1, Thomas Moritz 3, Stefan Jansson 2 and Johan Trygg 1

1 Research group for Chemometrics, Department of Chemistry, Umeå University SE-901 87 Umeå, Sweden
2 Umeå Plant Science Centre, Department of Plant Physiology, Umeå University SE-901 87 Umeå, Sweden
3 Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences SE-901 83 Umeå, Sweden

*To whom correspondence should be addressed.

Summary: MASQOT-GUI provides an open-source, platform-independent software pipeline for two-channel microarray spot quality control. This includes gridding, segmentation, quantification, quality assessment and data visualization. It hosts a set of independent applications, with interactions between the tools as well as import and export support for external software. The implementation of automated multivariate quality control assessment, which is a unique feature of MASQOT-GUI, is based on the previously documented and evaluated MASQOT methodology. Further abilities of the application are outlined and illustrated.

Availability: MASQOT-GUI is Java-based and licensed under the GNU LGPL. Source code and installation files are available for download at http://masqot-gui.sourceforge.net/

Contact: max.bylesjo{at}chem.umu.se

Supplementary information: Supplementary data are available at Bioinformatics online


Received on May 29, 2006; revised on July 7, 2006; accepted on August 4, 2006

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