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© 1987 IRL Press Ltd.

A non-linear regression program in BASIC for estimating Km and Vmax

Andrew Page

Department of Biological Sciences, Sheffield City Polytechnic Sheffield SI IWB, UK

This paper gives a program in BASIC for calculating the kinetic parameters Km and Vmax for an enzyme reaction from a set of paired values of reaction velocity at given substrate concentrations. An initial estimate of the two parameters is made using a weighted linear regression, these values are then used in an iterative process to fit the data to the Michaelis–Menten equation and give final values of Km and Vmax with their associated standard errors.

Received on July 15, 1986; accepted on November 13, 1986

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