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© 1987 IRL Press Ltd.

A program for the graphic representation and manipulation of DNA sequences

N. J. Moote , P. Chénier , M. Mikhail and J. Drouin *

Institut de recherches cliniques de Montréal 110 avenue des Pins ouest, Montréal, Québec H2W 1R7, Canada

*To whom reprint requests should be sent.

We have developed a program for the graphic representation and manipulation of DNA sequences. The program (named CARTE from the French for ‘map’) is intended as a tool in the planning and analysis of recombinant DNA experiments. DNA sequences are represented as standard restriction maps, using any desired combination of restriction enzymes. Features of interest, such as promoters or coding sequences, can be highlighted. The sequence can be manipulated to mimic cloning, using deletions, insertions or replacements at specified sites. This process is facilitated by the simultaneous display of a graphic map of the entire sequence, a detailed picture of the work in progress, and a menu of functions.

Received on November 17, 1986; accepted on March 12, 1987

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