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© IRL Press Limited

A Macintosh Hypercard stack for calculation of thermodynamically-corrected buffer recipes

Robert J. Beynon

Department of Biochemistry, University of Liverpool PO Box 147, Liverpool L69 3BX, UK E.mail: SB6{at}UK.AC.LIVERPOOLIBM (JANET & BITNET)

This paper describes a stack for the Apple MacintoshlTM, HypercardTM environment that facilitates the calculation of a set of thermodynamically-corrected pH buffers. Presented in the familiar Macintosh mouse-based environment, the program allows comprehensive buffer design and gives the user full control of buffer species, temperature, ionic strength, pH and choice of counter-ion to maintain the ionic strength at a fixed value. Addition of new buffers to the stack is straightforward and the recipes that are generated, including the weights of the various components, can be displayed on the screen or saved to a disk file for subsequent printing.

Received on May 18, 1988; accepted on July 15, 1988

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