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© IRL Press Limited

SED88: a Pascal program for the analysis of sedimentation equilibrium data

P. M. Reid , A. E. Wilkinson , K. C. Leung 1 and M. N. Jones *

Department of Biochemistry and Molecular Biology, University of Manchester Manchester MI3 9PT
1Department of Electrical Engineering and Electronics, University of Manchester Institute of Science and Technology PO Box 88, Manchester M60 IQD, UK

*To whom reprint requests should be sent

Analytical ultracentrifugation is commonly used for the determination of molecular weights (sedimentation equilibrium) and sedimentation coefficients (sedimentation rate) of biological macromolecules in solution. A Turbo Pascal program for the analysis of sedimentation equilibrium centrifugation data produced by absorbance optical systems is described. The user may enter data from a scan of absorbance versus distance from the centre of rotation, via a graphics tablet (or ASCII file). This is subsequently manipulated to yield an apparent weight average molecular weight for the given sample. Plots of In (absorbance) versus (radius2) may also be produced. The method described uses readily available computational equipment requiring only a graphics tablet in addition to an IBM PC compatible computer. This technique and the software developed have been used to investigate the molecular weight range of two International Humic Substances Society (IHSS) reference samples from the Suwannee River.

Received on October 7, 1988; accepted on December 12, 1988

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