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© IRL Press

Automatic computation of enzyme kinetics by HPLC

Pier Luigi Mauri , Maria Bitonti 1, Mario Pace *, Pier Giorgio Pietta and Dario Agnellini

Dipartimento di Scienze e Tecnologie Biomediche, Sez. Chimica Organica, University of Milano via G.Celoria 2, 20133 Milano
1Ing, C. Olivetti & Co. S.p.A. Ivrea, Italy

*To whom reprint requests should be sent.

Enzyme activity can be easily measured by HPLC using traces of the product itself as an internal standard. Our procedure involved the development of an equation using the experimental data obtained in the kinetic assay. The entire procedure can thus be automated and a computer program is presented here for facilitating the assay and saving time. The determination of the activity of NAD kinase is reported as an example.

Received on March 15, 1990; accepted on May 17, 1990

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