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© IRL Press

POLLUX: a program for simulated cloning, mutagenesis and database searching of DNA constructs

Henry E. Dayringer and Scott A. Sammons

Monsanto Company 700 Chesterfield Village Parkway, Chesterfield, MO 63198, USA

Computer support for research in biotechnology has developed rapidly and has provided several tools to aid the researcher. This report describes the capabilities of new computer software developed in this laboratory to aid in the documentation and planning of experiments in molecular biology. The program, POLLUX, provides a graphical medium for the entry, edit and manipulation of DNA constructs and a textual format for display and edit of construct descriptive data. Program operation and procedures are designed to mimic the actual laboratory experiments with respect to capability and the order in which they are performed. Flexible control over the content of the computer-generated displays and program facilities is provided by a mouse-driven menu interface. Programmed facilities for mutagenesis, simulated cloning and searching of the database from networked workstations are described.

Received on January 23, 1990; accepted on October 31, 1990

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