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© IRL Press

GELYMAC: a Macintosh application for calculating DNA fragment size from gel electrophoresis migration data

Peter J. Russell , Joshua M. Doenias and Steven J. Russell

Biology Department, Reed College Portland, OR 97202, USA

A program called GELYMAC takes data on the distances migrated by DNA fragments in a one-dimensional electro-phoretic gel and, using a cubic-spline best-fit of marker fragment distance migrated versus molecular size, calculates the molecular sizes of the fragments. Written in the Rascal (Realtime Pascal) programming language, the program runs on the Macintosh family of microcomputers. Rapid entry of marker and experimental fragment migration data is afforded using a scroll bar system adjacent to a graphic representation of a gel. Output includes tabular listing of the data, graphic cartoons of the gel, and the fragment locations and molecular sizes for individual gel lanes, and the calibration curve used in data computations.



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