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© Oxford University Press

Using fragment lengths from incomplete digestion by multiply cleaving enzymes to map antibody binding sites on a protein

R. A. Sutherland and M. D. Partis 1,2

Horticulture Research International Wellesbourne, Warwick CV35 9EF
1Horticulture Research International Worthing Road, Littlehampton, West Sussex BN17 6LP, UK

2To whom reprint requests should be sent

The problem of mapping the positions of the unique binding sites of several monoclonal antibodies on a linear protein structure is considered. Data giving the incidence of binding of individual antibodies to fragments of the protein obtained from it by the incomplete chemical or enzymatic digestion are used to formulate a series of linear programming problems. The solution to these problems shows which orderings of binding sites are possible, and gives upper and lower bounds for the relative positions of the sites.

Received on January 20, 1991; accepted on July 22, 1991

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