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© Oxford University Press

Analysis of context of 5'-splice site sequences in mammalian mRNA precursors by subclass method

Mineichi Kudo , Sumie Kitamura-Abe , Masaru Shimbo and Yôichi lida 1

Department of Information Engineering, Faculty of Engineering Japan
1Department of Chemistry, Faculty of Science, Hokkaido University Sapporo, 060, Japan

The signals that direct the excision of introns from mammalian pre-mRNA are not yet well understood. However, at least three lands of signals—5'-splice site signals, 3'-splice site signals and branch point signals;—play important roles in the excision of introns. In the present paper we treat only the 5'-splice sites. In addition to a consensus sequence for 5'-splice signals, several methods have been proposed, based on a statistical model, and used to analyze relative importance of each nucleotide at each position. In our approach a nucleotide sequence is regarded as a string with symbols of ‘A’, ‘T’ ‘G’ and ‘C’; important substrings of 5'-splice site sequences, called pattern sequences, are extracted. A pattern sequence expresses which nucleotide is needed at a limited number of positions around the 5'-splice site. It is observed that a particular pattern sequence matches predominantly 5'-splice site sequences nearest to the 5'-end of a gene and another pattern sequence matches predominantly the second nearest ones. Moreover, it is confirmed that the pattern sequences accurately predict authentic 5'-splice sites for unknown genes and explain some mutation examples.

Received on October 3, 1991; accepted on January 14, 1992

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