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© Oxford University Press

DIROM: an experimental design interactive system for directed mutagenesis and nucleic acids engineering

Kira S. Makarova , Alexander V. Mazin , Yury I. Wolf 1 and Victor V. Soloviev 2

Institute of Cytology and Genetics, Siberian Division of the Academy of Sciences of USSR Novosibirsk 630090, USSR
1Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of USSR Novosibirsk 630090, USSR

2 To whom correspondence should be addressed

A computer system DIROM for oligonucleotide-directed mutagenesis and artificial gene design has been designed for better experimental planning and control. DIROM permits searching for optimal oligonucleotides with respect to certain important parameters, namely sufficient energy of oligonucleotide-target hybridization, the secondary structure of oligonuc-tide and target DNA, the presence of alternate binding sites in the target DNA and terminal G/C pairs. It can also be used to plan polymerase chain reaction experiments, for optimal primer selection, in sequencing, etc. DIROM enables one to search for both existing and potential restriction sites, to perform vector + target sequence construction. The system consists of a set of original algorithms that formalize the empirical knowledge of oligonucleotide action as primers.

Received on April 20, 1991; accepted on January 1, 1992

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