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© Oxford University Press

Time-optimized analysis of slit-scan chromosome profiles on a general-purpose personal computer

Rainer Heilig 1, Michael Hausmann 1, Wim Rens 2, Jacob A. Aten 2 and Christoph Cremer 1,3,4

1Institute of Applied Physics of the University Albert-Überle-Strasse 3-5, D-69120 Heidelberg, Germany
2Laboratory of Radiobiology Gebouw F-0, AMC, Meibergdreef 9, NL-1105 AZ Amsterdam, The Netherlands
3Interdisciplinary Centre for Scientific Computing (IWR) of the University Im Neuenheimer Feld 368, D-69120 Heidelberg, Germany FRG

4To whom reprint requests should be sent

Slit-scan flow cytometry provides a method to analyze large numbers of metaphase chromosomes in a relatively short time due to morphological features. The high detection rate requires fast computing for on-line analysis. Up to now, this has been achieved using special-purpose computers, parallel systems or other complex hardware. Here, we describe an algorithm that can be implemented on a general-purpose personal computer. Digitized chromosome profiles can be classified by several criteria especially for the detection of chromosome abnormalities in biological dosimetry. A data set of {bsim}4600 profiles was used. Programming in assembler results in an average computing time of about 600 µs per profile. Thus on-line evaluation of slit-scanning data appears to become feasible for many flow cytometers running nowadays.

Received on March 18, 1992; accepted on June 5, 1992

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