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© Oxford University Press

A computer program to calculate and design oligonucleotide primers from amino acid sequences

John H.E. Nash

Institute for Biological Sciences, National Research Council of Canada Ottawa, Ontario, Canada K1A 0R6

A computer program to generate oligonucleotide sequences from peptide data is presented. The program, PrimerGen, reverse-translates a peptide string into its corresponding degenerate deoxyribonucleotide counterpart, and generates sequences of oligonucleotide molecules which can be synthesized and either used as probes for hybridizations or as primers in polymerase chain reactions.

; accepted on October 15, 1992

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