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© Oxford University Press

STADEERS: a software package for the statistical design of experiments pertaining to the estimation of parameters in rate expressions that describe enzyme-catalyzed processes

Victor M. Balcão and F.Xavier Malcata 1

Escola Superior de Biotecnologia, Universidade Cardlica Portuguesa, Rua Dr AntOnio Bernardino de Almeida 4200 Porto, Portugal

1To whom correspondence should be addressed

This paper describes a computational algorithm (STADEERS-STAtistical Design of Exeriments in Enzyme ReactorS) for the statistical design of biochemical engineering experiments. The type of experiment that qualifies for this package involves a batch reaction catalyzed by a soluble enzyme where the activity of the enzyme decays with time. Assuming that both the catalytic action and the deactivation of the enzyme obey known rate expressions, the present code is helpful in the process of obtaining estimates of the kinetic parameters by providing as output the times at which samples should be withdrawn from the reacting mixture. Starting D-optimal design is used as a basis for the statistical approach. This BASIC code is a powerful tool when fitting a rate expression to data because it increases the effectiveness of experimentation by helping the biochemical kineticist obtain data points with the largest possible informa tional content.

Received on March 12, 1992; accepted on December 1, 1992

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