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Bioinformatics Advance Access published online on June 9, 2008
Bioinformatics, doi:10.1093/bioinformatics/btn283
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© The Author (2008). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Sequence-specific reconstruction from fragmentary databases using seed sequences: implementation and validation on SAGE, proteome and generic sequencing data

Tiago J.P. Sobreira 1 and Arthur Gruber 2,*

1Instituto do Coração - USP, Av. Prof. Enéas de Carvalho Aguiar 44, Bloco 2, 10o andar, 05403-000, São Paulo SP, Brazil.
2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo SP, 05508-000, Brazil.

*To whom correspondence should be addressed. Prof. Arthur Gruber, E-mail: argruber{at}usp.br


  Abstract

Motivation: DNA assembly programs classically perform an all-against-all comparison of reads to identify overlaps, followed by a multiple sequence alignment and generation of a consensus sequence. If the aim is to assemble a particular segment, instead of a whole genome or transcriptome, a target-specific assembly is a more sensible approach. GenSeed is a Perl program that implements a seed-driven recursive assembly consisting of cycles comprising a similarity search, read selection and assembly. The iterative process results in a progressive extension of the original seed sequence. GenSeed was tested and validated on many applications, including the reconstruction of genomic nuclear genes or segments, full-length transcripts, and extrachromosomal genomes. The robustness of the method was confirmed through the use of a variety of DNA and protein seeds, including short sequences derived from SAGE and proteome projects.

Availability: GenSeed is available under the GNU General Public License at http://www.coccidia.icb.usp.br/genseed/

Contact: argruber{at}usp.br

Supplementary information: http://www.coccidia.icb.usp.br/genseed/

Associate Editor: Prof. John Quackenbush

Received on December 13, 2007; revised on May 25, 2008; accepted on June 8, 2008

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