Bioinformatics Advance Access originally published online on November 10, 2006
Bioinformatics 2007 23(2):134-141; doi:10.1093/bioinformatics/btl565
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Predicting transcription factor affinities to DNA from a biophysical model
Max-Planck-Institute for Molecular Genetics Ihnestrasse 73, 14195 Berlin, Germany
*To whom correspondence should be addressed.
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ABSTRACT |
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 TOP ABSTRACT 1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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Motivation: Theoretical efforts to understand the regulation of gene expression are traditionally centered around the identification of transcription factor binding sites at specific DNA positions. More recently these efforts have been supplemented by experimental data for relative binding affinities of proteins to longer intergenic sequences. The question arises to what extent these two approaches converge. In this paper, we adopt a physical binding model to predict the relative binding affinity of a transcription factor for a given sequence.
Results: We find that a significant fraction of genome-wide binding data in yeast can be accounted for by simple count matrices and a physical model with only two parameters. We demonstrate that our approach is both conceptually and practically more powerful than traditional methods, which require selection of a cutoff. Our analysis yields biologically meaningful parameters, suitable for predicting relative binding affinities in the absence of experimental binding data.
Availability: The C source code for our TRAP program is freely available for non-commercial use at http://www.molgen.mpg.de/~manke/papers/TFaffinities/
Contact: vingron{at}molgen.mpg.de
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1 INTRODUCTION |
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TOPABSTRACT1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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Protein–DNA interactions play a fundamental role in transcriptional gene regulation. For individual sequences, these interactions have been studied for a long time using a variety of experimental techniques, such as DNAse footprinting (Galas and Schmitz, 1978) and gel-shift assays (Fried and Crothers, 1981). Recently, functional genomics technology has opened up the way towards unraveling protein–DNA interactions on a global scale. In particular the group of Rick Young has pioneered the genome-wide application of chromatin-immuno precipitation for a comprehensive list of transcription factors in Saccharomyces cerevisiae (Lee et al., 2002; Harbison et al., 2004). In this technique the bound and unbound sequence fragments are labeled with red and green dye respectively and are then simultaneously hybridized onto an array (ChIP-chip). The relative intensities from the two channels (R/G ratios) provide a quantitative estimate for the binding affinities of a transcription factor to all sequence regions of interest in vivo. Generally, the measured affinities depend on the cellular condition in which the binding of the transcription factor (TF) is tested. Such alterations can be due to differences in protein concentrations and DNA accessibility. Therefore a complementary approach of protein binding microarrays (PBMs) has been developed by Martha Bulyk and her collaborators (Mukherjee et al., 2004). It allows to quantify the relative affinities of a TF to accessible, double-stranded DNA in vitro, again in terms of R/G ratios.
Where experimental data do not suffice yet to determine e.g. whether a particular transcription factor binds a target gene, theoretical considerations have to fill the gap. The groundbreaking work by von Hippel and Berg (1986) provided the rationale for converting the biophysical problem of TF–DNA affinity into a pattern matching and pattern discovery problem (Stormo 2000; D'haeseleer, 2006; Djordjevic et al., 2003). Following these ideas, the preferential binding of some TFs to certain DNA sequences can be expressed in terms of a sequence motif or a position specific score matrix (Wasserman and Sandelin, 2004), which is derived from a set of known high-affinity binding sequences. For a stretch of DNA, such a description assigns a score to every site in the sequence depending on its similarity to the motif. Traditionally, statistical considerations are then used to define a score threshold which needs to be exceeded in order for a site to be reported as a hit (Rahmann et al., 2003). Such hit-based methods cement the binary separation between binding and non-binding, in contrast to the physical behavior of TFs. It has therefore been difficult to rationalize the binding affinities measured with the ChIP-chip and PBM technologies using the motif-matching approach described above.
In this paper, we put forward a method for predicting the binding affinity of a TF to a DNA sequence of interest. Our probabilistic framework is closer in spirit to the original work by Berg and von Hippel (1987) and circumvents the need for a threshold on both the experimental data and our predictions. As a measure of relative affinity we use the expected number of TFs bound to a DNA sequence. Our TRanscription factor Affinity Prediction (TRAP) tool which calculates this quantity takes as input a matrix description of a given TF and a set of DNA sequences to be annotated. It requires the specification of only two parameters, 
and R0. Here we draw upon the large scale ChIP-chip and PBM datasets to calibrate these two parameters. Strikingly, the relative binding affinities predicted by TRAP are rather insensitive even to sizeable variations in the parameter values and reveal interesting information about the driving forces in protein–DNA interactions. This enables us to provide a general prescription for and R0. Once tuned, this model allows the prediction of relative binding affinities also in the absence of large-scale binding data.
Recent work by Tanay (2006) has also advocated an affinity-based approach to TF binding. He and also Foat et al. (2006) have developed methods, to derive optimal scoring matrices for individual TFs given binding data from ChIP-chip. Our aim is not to derive TF representations, which correlate optimally with ChIP-chip data, but rather to optimize a generic physical model which can rationalize the binding data for all TFs. This is also in contrast to Granek and Clarke (2005), who utilize a physical model, but do not provide a rationale for choosing the parameters.
Our approach of predicting binding affinities has a number of advantages over traditional hit-based methods. Most notably, TRAP provides a natural ranking of sequences with respect to a particular TF of interest or conversly the ranking of several TFs with respect to one sequence. Finally, we compare our results with traditional approaches and find that it has higher predictive power over experimental binding ratios than the hit-based methods.
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2 METHODS |
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TOPABSTRACT1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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2.1 Protein–DNA binding data
In this work we utilize the genome-wide dataset on in vivo protein–DNA interactions in S.cerevisae (Harbison et al., 2004). The authors provide a list of binding ratios (R/G-ratios) for all intergenic regions in yeast, which we obtained from their website. For comparison, we also retrieved binding data for three TFs (Rap1, Mig1 and Abf1) from a complementary study by Mukherjee et al. (2004), who use protein binding microarrays to determine binding affinities in vitro. For each dataset the authors suggest a P-value threshold of 0.001 to discriminate between binding and non-binding which we utilize for parts of the analysis.
2.2 Binding site descriptions
As motif descriptions we use the set of 29 curated yeast matrices (for 25 factors) provided by the TRANSFAC database (Matys et al., 2003) for which ChIP-chip data are available. We add a pseudo-count of 
= 1 to each element in the count matrices (Bucher, 1990). This modification can be interpreted in statistical terms as setting the estimated number of unobserved base pair occurrences, or physically, as setting a maximally allowed contribution to the mismatch energy. For comparative purposes we also set = 0.5, but our results are unaffected by such a change.
2.3 A simple model of protein–DNA interactions
Assuming that the complex formation of a transcription factor TF with a sequence site S is at equilibrium, TF + S 
TF · S, the fraction of bound sites is given by Zumdahl (1998)
 | (1) |
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and ßE(S) explicit. Berg and von Hippel showed that the mismatch energy, E(S), can be written in terms of a TF-specific matrix, M = (mi,
), which summarizes the observed base pair counts of known TF binding sites Berg and von Hippel (1987).
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and 
only if the sequence has base pair at position i, and zero otherwise. For each position the matrix element with maximal count is denoted as mi,max. This also defines the consensus sequence, for which every term in the above sum vanishes, such that ßE(S) = 0. Finally, we include a background-dependent term, 
, which denotes the relative background frequency of the observed nucleotide with respect to the background frequency of the most frequent nucleotide in the motif at the given position. For a given TF, Equation (4) effectively replaces the large set of unknown binding energies, ßE(S), by a predefined motif matrix and a single parameter , which is introduced to scale the mismatch energies in units of thermal energy. This parameter depends on the TF of interest and determines how strongly variations in the target sequence will be penalized. This completes the reduction of the large parameter space to only two sequence-independent parameters (R0,).
As a measure of relative affinity our TRAP program predicts for a given TF matrix of length W and a given DNA sequence of length L the expected number 
N
of bound transcription factor molecules. This quantity is computed as the sum of contributions from all possible sites l in the sequence of interest
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), we used
 | (6) |
are large, i.e. for palindromic motifs. In general one could invoke more elaborate dynamic programming techniques, as used by Rajewsky et al. (2002), to account for preclusion effects from competing factors and self-overlapping binding sites. However, such effects will be small for our analysis, in which we treat all TFs separately. They are likely to be more pronounced when multiple TFs compete for the same sequence. We leave such a treatment to future analysis (Chung et al., manuscript in preparation).
Parameter determination
To calibrate the parameters R0 and for a given TF and cellular condition we apply Equation (5) to all 6700 intergenic regions in yeast. This results in 6700 predicted occupancies N, which can be correlated with the measured R/G ratios from ChIP-chip experiments. We use the Pearson correlation coefficient, r, to quantify how well the model describes the experimental binding affinity and to determine the optimal parameters. To assume a linear correlation between the R/G ratios and N is plausible if the efficiency of the pulldown reaction (ChIP) is small and not yet saturated. This is supported by the absence of any apparent upper limit on the measured R/G ratios. We have tested the range of parameter values = 0.05, 0.10, ..., 2.00 and ln R0 = –10, –8, ..., 30 for all TFs and all tested conditions. We take those parameters which yield the highest correlation coefficient as optimal, in the sense that with this choice of R0 and the model describes the actual binding data best.
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3 RESULTS |
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TOPABSTRACT1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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3.1 Screening the parameter space
As a measure of affinity, TRAP predicts for a given TF and a given DNA sequence the expected number,
N, of bound TF molecules. This calculation requires the setting of two parameters. The first parameter, R0, involves the factor concentration and the equilibrium constant of the binding reaction between the TF and its optimal binding site. The second parameter, , scales the mismatch energy of a given site in the sequence with respect to the optimal binding site. For any given combination of R0 and the correlation between N of every intergenic region and the corresponding experimental R/G ratios can be determined.
First we analyze the generic features of the correlation coefficient across the parameter space spanned by R0 and . These features are illustrated by the example shown in Figure 1. For large (small mismatch energy) as well as for large R0 almost every site in the sequence is occupied and the number of expected TFs bound will simply correlate with the length of the intergenic region. This typically results in a poor correlation with the observed R/G-ratios as can be seen in the upper right part of Figure 1.
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For small
(large mismatch energy) only the optimal sites, E = 0, will have a non-vanishing binding affinity. However, most TFs can accommodate certain variations in the binding site (Mossing and Record, 1985), therefore in most cases we would not expect to observe the best correlation with experimental data in a region of the parameter space which does not permit a certain degree of binding site flexibility. This is in line with our observation in Figure 1, where the correlation coefficient decreases for 
0.
For small R0 the expected number of bound TFs depends linearly on R0, as can be inferred from a Taylor expansion of Equation (5) around R0 = 0
 | (7) |
N, but not the correlation of N with R/G ratios. In Figure 1 this is reflected by a constant correlation coefficient for ln R0 < 0 and a given .
It is evident from Equation (5) that the affinity of a single binding site can be kept constant for varying values of R0 and ßE in such a way that ln R0 – ßE = c. With ßE 
1/, we find the hyperbolic relation 1/(ln R0 – c). Interestingly the characteristic curves of constant correlation coefficients seen in Figure 1, which can be well described by a hyperbola, suggest that this generic behavior is effectively reflected in the behavior of the correlation coefficients.
Optimal parameter choice derived from experimental data
Binding data from PBM constitutes the ideal benchmark for TRAP. We thus determined the optimal model parameters for Abf1, Mig1 and Rap1 whose binding affinities have been studied experimentally using protein binding arrays (Mukherjee et al., 2004) and for which we obtained matrix descriptions from TRANSFAC (Matys et al., 2003). In all cases we can find optimal parameters which yield highly significant correlation (r > 0.5), as shown in Table 1. This indicates that TRAP can successfully account for much of the observed in vitro binding affinities.
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We proceed to a more comprehensive set of 25 TFs (29 matrices), for which matrix descriptions exist (Matys et al., 2003) and R/G ratios have been obtained from ChIP-chip for one or more cellular conditions (Harbison et al., 2004). This in vivo data corresponds to a more complicated situation, where we cannot always assume that the TF is available for DNA binding and that the DNA is accessible under the tested condition. Despite these caveats, we observe that TRAP still predicts a large fraction of in vivo affinities for properly chosen parameters. In Table 1 we present our results for a group of 15 matrices for which our affinity predictions show high correlation (Pearson r > 0.3) with the experimentally observed R/G ratios. We provide a complete list for all 25 factors and 13 conditions as Supplementary table.
Remarkably, the optimal parameters for all factors and conditions correspond to maximal values of N (over all intergenic regions) in the range of 0.5...5. This is biologically reasonable assuming that each transcription factor should recognize some promoter region, at least in one condition. Nmax falls outside of this range only in the case of Hap1, where the ‘optimal’ R0 is small and poorly defined in the sense explained below Equation (7), and Rap1, where several sequences have large clusters of neighboring Rap1 binding sites (Gilson et al., 1993).
Notice that the observed correlations are actually quite insensitive to the precise value of the parameters and some of our modeling assumptions. We also investigated the rank order of different intergenic regions with respect to their predicted affinities. While the absolute value for N depends on the values of (ln R0, ), we find that the ranking of intergenic regions remains largely unaffected even under sizeable changes in these parameters. Comparing the ranks of the TRAP results for optimal parameters with those obtained from a 30% decrease in , we find Spearman rank correlation coefficients larger than 0.98. Similarly, an almost 100-fold change in R0 gave a correlation coefficient above 0.99.
Parameter choice in the absence of experimental data
While it is possible to determine the optimal coefficients (R0, ) in the presence of sufficient binding data, it is clearly desirable to have some prescription which would allow the parameter determination on general grounds. Based on the results in Table 1 and the observed insensitivity to small changes in the parameters we decided to fix to an average value of 0.7 for all TFs and all conditions. This fixation reduces the parameter space to only R0. We observe that the optimal values of R0( = 0.7) can be well described as a function of the motif width, W, with only small changes due to condition dependent effects. This is shown in Figure 2 where we perform a regression analysis of ln R0 against W. The regression line allows us to determine R0 for any given W and provides the basis for our subsequent analysis. R0 will also vary with the cellular condition, through changes in TF-concentration, but empirically we find that this amounts to much smaller shifts compared to the overall dependence on the motif length. This can be understood since R0 depends only linearly on the concentration, [TF], but exponentially on the difference between the free energies of the best binding complex and the unbound state. This difference increases with the width of the binding site through an increasing number of protein–DNA contacts and dominates the behavior of R0 as shown in Figure 2.
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It should be noted that matrices can contain unspecific positions which then define an arbitrary consensus site with spuriously low binding energy. This can lead to an overestimate of the ‘optimal’ R0 as observed in case of GCN4_01 (27 bp). For identical
, GCN4_01 gives a vastly larger estimate of R0 compared to GCN4_C (10 bp). The problem could be addressed by restricting the motif to positions with higher information content (e.g. 
0.2 bits). For GCN4_01 this would reduce the motif length to 11 bases and in turn improve the results for this matrix (maximal r 
0.57 and rpredicted 0.53 compared to the values in Table 1). Although the regression in Figure 2 could be further improved by these corrections we find that it is not very sensitive to such influences and in the following we thus proceed by using only the unmodified TRANSFAC matrices.
Assuming the parameter prescription 
, we find correlations with the R/G-ratios that are almost as high as the optimal correlations (last column of Table 1) with exception of GCN4_01. This choice of (R0, ) may be used to predict relative binding affinities for TFs with known motifs in the absence of genome-wide binding data.
Comparison of TRAP with hit-based methods
Traditionally, computational target predictions have focused on the identification of individual binding sites with more or less specific sequence patterns. This is usually done by scanning a score matrix along the sequence and assigning a ‘hit’, whenever the score exceeds some pre-defined threshold (Wasserman and Sandelin, 2004). Of course, traditional methods suffer from the arbitrariness of that threshold. In contrast, our focus is on the determination of relative binding strength and the expected number of bound TFs. Therefore, it is difficult to compare our affinity-based method and hit-based methods without adjusting one or the other.
Here we consider two commonly used hit-based methods and compare them to TRAP [using the predefined parameters R0(W), = 0.7] with respect to their capability of predicting experimental binding ratios. The first traditional method, which we call ‘balanced method’, invokes a score threshold which is chosen such that the expected number of false positive hits is balanced by the expected number of false negatives (Rahmann et al., 2003). For each sequence this method calculates a number of hits which can be compared to experimental binding ratios and our predictions for the expected number of bound TFs. This comparison is illustrated for Leu3 in Figure 3, where it can be seen that the TRAP approach leads to a better correlation with experimental data. For a second comparison, we also consider a different threshold prescription, called ‘5FP’, in which the expected false-positive rate is arbitrarily set to 5%. In Table 2 we provide a complete comparison of all the methods described above.
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It can be seen that in
80% of cases TRAP results in better correlations with experimental binding ratios than the hit-based methods.
Alternatively, one may also impose a cutoff on the expected counts N to (arbitrarily) discriminate between bound and unbound sequences. Also experimental binding data are often interpreted in such a binary way, where binding ratios are converted to P-values and only sequences with e.g. P < 0.001 are considered as bound (Harbison et al., 2004). For varying thresholds on N and a given cutoff on R/G ratios we can then calculate different sensitivities and specificities which are evaluated in a ROC-curve analysis. In Table 2 we use the area under the ROC-curve as a quality measure. Most areas are much larger than 0.5, indicating a strong predictive power of this method over experimental binding data in yeast at the significance threshold of P < 0.001.
To compare again with hit-based methods, we took, for each TF and every intergenic region, the number of hits (for 5FP and balanced cutoff) and performed the same ROC-curve analysis based on different thresholds on this score. Again we find that our method performs consistently better than hit-based approaches (see Table 2). On the entire set of 29 matrices we find that TRAP yields a ROC curve area of 0.7 for 22 matrices in at least one of the experimentally tested condition as opposed to only 16 and 14 matrices for the balanced and 5FP cutoff methods, respectively (see Supplementary Table).
Prediction of TFs with high affinity
The above analysis shows that for a given TF we can successfully rank sequences according to their expected affinity. Here we address the complementary question: given a certain sequence, can TRAP successfully rank TFs in accordance with ChIP-chip experiments using our prescription R0(W), = 0.7. In general factors bound to a given sequence in the ChIP experiment should have higher values of N predicted by TRAP than unbound factors. Since experimental R/G ratios for different factors are not directly comparable, we follow again the binding prescription as given in (Harbison et al., 2004; Mukherjee et al., 2004) and distinguish binders from non-binders according to the P-value threshold of P = 0.001.
Figure 4 shows as an example the intergenic region between GAL1 and GAL10 with its experimentally verified high affinity sites for Gal4 and Mig1 (Selleck and Majors, 1987; Frolova et al., 1999). This region was also significantly enriched in the ChIP-chip pulldown experiment with GAL4 and in the PBM experiment with MIG1. None of the other 23 factors in our set had been predicted as a target by ChIP-chip or PBM. As can be seen, TRAP predicts the highest affinities for Gal4 followed by Mig1, Adr1 and Ste12. All other factors have only negligible affinities predicted in good agreement with the ChIP-chip experiments. Interestingly independent chromatin precipitation experiments have shown that Ste12 has weak but measurable affinity to the GAL1–GAL10 intergenic region (Reeves and Hahn, 2005). The balanced cutoff method also predicts these four factors as potential binders but in addition four others (Ap1, Gcr1, Hsf1 and Rox1). If one ranks traditional annotations according to the number of hits, then Gal4 is ranked highest with seven annotated hits followed by Adr1 with two while Mig1 and Ste12 with one binding site each are assigned a tied rank with Ap1, Gcr1, Hsf1 and Rox1.
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This analysis was carried out on the entire set of 4451 intergenic sequences which have a ChIP-chip P-value assigned for all our 25 factors. In total this sequence set yields 2388 significant TF–DNA interactions with P < 0.001. To assess the quality of different TF ranking schemes we count for each sequence the number of bound TFs ranked above all unbound TFs. For TRAP, TFs are ranked according to
N and for the hit-based methods according to the number of annotated hits as described for the example above. In those cases where several factors have the same number of hits annotated but only a subset of the factors correspond to bound factors, we determine, based on the average of 1000 random samplings, how many times the unbound TFs will accidentially be ranked above a given bound TF. The analysis shows that 643 (27%) of the significant interactions are correctly ranked on top according to TRAP as compared to 343 (14%) in case of the balanced cutoff and 551 (23%) in case of the 5FP method. These results show that in a considerable number of cases the ranking of TFs according to TRAP is in accordance with ChIP-chip data and overall better than traditional hit-based methods.
Contributions from low affinity sites to N
While our method predicts the overall affinity of a transcription factor to a sequence region, it is still possible to ask which sites contribute most significantly to this affinity. Here we study in more detail the relative contribution of different sites to the total expected count, N, and therefore to the correlation of N with the observed binding ratios. To this end, we rank all sites in a given sequence according to their probability of being bound, p(Sl), and approximate the expected number of bound TFs by the sum of its n top-ranking sites, 
. This analysis is illustrated in Figure 5 for Leu3.
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to only the top n scoring terms then the expected counts, We find that for the majority of matrices a better correlation can be obtained when all sites are taken into account rather than a single strongest site. This suggests that the relative binding affinities for a given intergenic region are well modeled by taking the total sum over all sites in the region, and supports our claim that a mechanistic description of binding data is possible without imposing any threshold.
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4 DISCUSSION |
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TOPABSTRACT1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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We have applied a physical model to predict the relative binding affinities of TFs to regulatory regions of the DNA. In contrast to the traditional search for binding sites, we do not impose any threshold, but integrate the contributions from individual strong sites and weak sites to calculate the expected number of bound TFs. The ranking of sequence fragments according to this affinity measure is robust with respect to sizable variations in the space of two parameters which define the binding model. Using recent in vitro and in vivo data from budding yeast, we find that
lies in the range of [0.4, 1.5] for most factors. The other parameter R0 is largely determined by the width of the binding site, and to a much lesser extent by the TF concentration. We provide a simple parameterization of the Berg-von Hippel model [ = 0.7, R0 = R0(W)] and show that a large fraction of our affinity predictions are significantly correlated with experimentally measured R/G ratios in one or more cellular conditions. Our results indicate that TRAP can better predict relative binding affinities than any of the hit-based approaches. This improvement is due to our probabilistic approach to binding affinities, which avoids assigning a discrete number of binding sites to a sequence. Moreover it takes into account contributions from weak sites and hence can assign affinities for sequences where hit-based methods fail to report any ‘match’. It also accounts for differences in the binding strength of sites which are traditionally only reported as hits. This is not only reflected in better correlation but also better and more robust ranking of TFs as compared to hit-based methods.
Considering a comprehensive list of 25 factors and 13 conditions (61 experimentally tested combinations), we find that our predictions resulted in high correlations (r > 0.3) for 23 of these combinations. In addition for 36 combinations TRAP yielded a ROC curve area 0.7. It is encouraging to see that our predictions also match what is known about the involvement of TFs in the various conditions tested. For example, Hsf1, Rcs1 and Leu3 are known to be involved in several aspects of stress response (Raitt et al., 2000; Blaiseau et al., 2001; Zhou et al., 1987) and their predicted affinities show high correlation with R/G ratios only in conditions of oxidative stress (H2O2) and amino acid starvation, but not in rich medium.
This also suggests why, for certain factors and cellular conditions, the physical model cannot be expected to predict binding affinities in vivo. Indeed, for nine factor-condition pairs with only small correlation (r < 0.3) the TFs may not be expressed or available for binding under the condition tested. These include Adr1, Hac1, Mata1, Pdr3, Pho4, Xbp1, Yap1 and Zap1 in rich medium and Mig1 in medium with galactose as carbon source. For example, Mig1 is known to be located in the cytoplasm in the presence of galactose, and hence it is not available for DNA-binding in the nucleus (Vit et al., 1997). However, our predictions for Mig1 do show a high correlation (r = 0.60) with in vitro data. Likewise, the binding ratios of Abf1 and Rap1 have also been determined in vitro and show a high correlation with our predictions (r > 0.5) under this condition. This is in accordance with our assumption that the TF is available and that the DNA is accessible.
The TRAP approach appears to fail for other matrices and conditions, even though we have no indication that the corresponding factor is absent. We want to stress that our approach requires the definition of matrix descriptions which can be used as good approximations for mismatch energies in the physical model. There are several cases where we suspect that the matrix description may be inappropriate. For example, for Hsf1 there are four matrices listed in TRANSFAC, but only one of them (an alternating trimer motif HSF1_04) yields good correlations with the experimental binding ratios. Interestingly the trimer combination of this matrix has been described as the site with highest affinity for Hsf1 (Sorger and Pelham, 1987; Xiao et al., 1991). It is possible that better predictions can be achieved by using improved matrices like in the case of GCN4_01 or matrices derived from ChIP-chip data (Foat et al., 2006; Tanay, 2006). The focus of this work, however, is to explain ChIP-chip data in a biophysical framework rather than the evaluation of matrices. Hence in the present study only publically available matrices are used.
The key ingredient of the model by Berg and von Hippel is the assumption that different basepairs contribute independently from each other to the overall binding energy. This assumption also entails that mismatch energies for large deviations from the consensus sequence are not calculated differently from small deviations. Since TF–DNA complexes can, presumably, compensate for the relative increase in free energy from base pair mismatches through other mechanisms, such as conformational changes the model may underestimate the binding affinity of weak sites and thus N.
The fact that already now a significant fraction of yeast binding data can be accounted for by matrix motifs is all but obvious given the complicated binding mechanisms in eukaryotes and the relatively simple energetic binding model. Prokaryotic binding data has triggered motif based models more than 20 years ago. Our results demonstrate that, despite the increased complexity of the eukaryotic cell, such energetic binding models are also of predictive value for yeast. It will be interesting to see to what extent the observations made for yeast will carry over to multi-cellular organisms.
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Acknowledgments |
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The authors would like to thank Ho-Ryun Chung, Stefan Haas, Philipp Messer, Peter Arndt and Roded Sharan for many valuable discussions and Szymon Kielbasa for providing help with the hit-based sequence annotation. This work was supported by a grant from the German National Genome Research Network (NGFN) and the EU (BioSapiens Network, contract number LSHG-CT-2003-503265).
Conflict of Interests: none declared.
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FOOTNOTES |
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Associate Editor: Chris Stoeckert
Received on October 1, 2006; revised on November 6, 2006; accepted on November 6, 2006
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REFERENCES |
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TOPABSTRACT1 INTRODUCTION2 METHODS3 RESULTS4 DISCUSSIONREFERENCES |
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Berg, O. and von Hippel, P. (1987) Selection of DNA binding sites by regulatory proteins. Statistical-mechanical theory and application to operators and promoters. J. Mol. Biol, . 193, 723–750[CrossRef][ISI][Medline].
Blaiseau, P., et al. (2001) Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast. J. Biol. Chem, . 276, 34221–34226
Bucher, P. (1990) Weight matrix descriptions of four eukaryotic RNA polymerase II promoter elements derived from 502 unrelated promoter sequences. J. Mol. Biol, . 212, 563–578[CrossRef][ISI][Medline].
D'haeseleer, P. (2006) What are DNA sequence motifs? Nat. Biotechnol, . 24, 423–425[CrossRef][ISI][Medline].
Djordjevic, M., et al. (2003) A biophysical approach to transcription factor binding site discovery. Genome Res, . 13, 2381–2390
Foat, B.C., et al. (2006) Statistical mechanical modeling of genome-wide transcription factor occupancy data by MatrixREDUCE. Bioinformatics, 22, e141–e149
Fried, M. and Crothers, D. (1981) Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res, . 9, 6505–6525
Frolova, E., et al. (1999) Binding of the glucose-dependent Mig1p repressor to the GAL1 and GAL4 promoters in vivo: regulation by glucose and chromatin structure. Nucleic Acids Res, . 27, 1350–1358
Galas, D. and Schmitz, A. (1978) DNAse footprinting: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids Res, . 5, 3157–3170
Gilson, E., et al. (1993) Distortion of the DNA double helix by RAP1 at silencers and multiple telomeric binding sites. J. Mol. Biol, . 231, 293–310[CrossRef][ISI][Medline].
Granek, J.A. and Clarke, N.D. (2005) Explicit equilibrium modeling of transcription-factor binding and gene regulation. Genome Biol, . 6, R87[CrossRef][Medline].
Harbison, C.T., et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature, 431, 99–104[CrossRef][Medline].
Lee, T.I., et al. (2002) Transcriptional regulatory networks in Saccharomyces cerevisiae. Science, 298, 799–804
Matys, V., et al. (2003) TRANSFAC: transcriptional regulation, from patterns to profiles. Nucleic Acids Res, . 31, 374–378
Mossing, M. and Record, M. (1985) Thermodynamic origins of specificity in the lac repressor-operator interaction. Adaptability in the recognition of mutant operator sites. J. Mol. Biol, . 186, 295–305[CrossRef][ISI][Medline].
Mukherjee, S., et al. (2004) Rapid analysis of the DNA-binding specificities of transcription factors with DNA microarrays. Nat. Genet, . 36, 1331–1339[CrossRef][ISI][Medline].
Rahmann, S., et al. (2003) On the power of profiles for transcription factor binding site detection. Stat. Appl. Genet. Mol. Biol, . 2, .
Raitt, D., et al. (2000) The Skn7 response regulator of Saccharomyces cerevisiae interacts with Hsf1 in vivo and is required for the induction of heat shock genes by oxidative stress. Mol. Biol. Cell, 11, 2335–2347
Rajewsky, N., et al. (2002) Computational detection of genomic cis-regulatory modules applied to body patterning in the early Drosophila embryo. BMC Bioinformatics, 3, 30[CrossRef][Medline].
Reeves, W.M. and Hahn, S. (2005) Targets of the Gal4 transcription activator in functional transcription complexes. Mol. Cell. Biol, . 25, 9092–9102
Selleck, S. and Majors, J. (1987) In vivo DNA-binding properties of a yeast transcription activator protein. Mol. Cell. Biol, . 7, 3260–3267
Sorger, P. and Pelham, H. (1987) Purification and characterization of a heat-shock element binding protein from yeast. EMBO J, . 6, 3035–3041[ISI][Medline].
Stormo, G. (2000) DNA binding sites: representation and discovery. Bioinformatics, 16, 16–23
Tanay, A. (2006) Extensive low-affinity transcriptional interactions in the yeast genome. Genome Res, . 16, 962–972
Vit, M.D., et al. (1997) Regulated nuclear translocation of the Mig1 glucose repressor. Mol. Biol. Cell, 8, 1603–1618[Abstract].
von Hippel, P. and Berg, O. (1986) On the specificity of DNA-protein interactions. Proc. Natl Acad. Sci. USA, 83, 1608–1612
Wasserman, W.W. and Sandelin, A. (2004) Applied bioinformatics for the identification of regulatory elements. Nat. Rev. Genet, . 5, 276–287[CrossRef][ISI][Medline].
Xiao, H., et al. (1991) Cooperative binding of Drosophila heat shock factor to arrays of a conserved 5 bp unit. Cell, 64, 585–593[CrossRef][ISI][Medline].
Zhou, K., et al. (1987) Structure of yeast regulatory gene LEU3 and evidence that LEU3 itself is under general amino acid control. Nucleic Acids Res, . 15, 5261–5273
Zumdahl, S.S. Chemical Principles, (1998) 3rd edn Houghton Mifflin Company.
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