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© IRL Press Limited

Computer-based learning of cooperativity and allostery

Rafael Franco and Enrique l. Canela *

Department of Biochemistry, Faculty of Chemistry, University of Barcelona Diagonal 647, 08028-Barcelona, Catalunya, Spain

*To whom reprint requests should be sent.

The aim of this article is to facilitate the understanding of enzyme cooperativity and allostery by undergraduate and postgraduate students with the aid of a graphic microcomputer. For this purpose the molecular models of Monod-Wyman-Changeux (MWC) and of Koshland-Nemethy-Filmer (KNF) are tested by showing how the different plots, direct, reciprocal, Scatchard and Hill, vary as do the parameters considered in these models. The programs used (one for each model) progress from easy aspects to complicated ones without the intervention of the user (student). Nevertheless, ultimately, with the MWC model, after the introduction of heterotropic effectors, the users can select the parameters in order to further their knowledge. This can be useful also for testing the kinetic behaviour of multisubunit enzymes which present cooperativity and which have been extensively described in the literature.

Received on March 28, 1985; accepted on June 20, 1985

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