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Bioinformatics Vol. 18 no. 2 2002
Pages 321-322
© 2002 Oxford University Press


Applications Note

Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays

H. B. Nielsen and S. Knudsen

Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark

Received on July 5, 2001 ; revised on October 10, 2001 ; accepted on October 19, 2001

Summary: PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3'end.

Availability: PROBEWIZ is accessible from http://www.cbs.dtu.dk/services/DNAarray/probewiz.html

Contact: hbjorn{at}cbs.dtu.dk; steen{at}cbs.dtu.dk


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