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Bioinformatics Advance Access originally published online on January 22, 2004
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Bioinformatics 20(3) © Oxford University Press 2004; all rights reserved.

Applications Note

Gene specific siRNA selector

Natasha Levenkova , Qingjuan Gu and John J. Rux *

The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA

Received on June 24, 2003 ; accepted on August 15, 2003
Advance Access Publication January 22, 2004

Summary: Small interfering RNA (siRNA) is used in functional genomics applications to decrease the expression of a target gene, which may yield a biological effect that suggests a function for the target gene. The siRNA design tool scans a target gene for candidate siRNA sequences that satisfy user-adjustable rules. Selected candidates are then screened to identify those siRNA sequences that are specific to the gene of interest.

Availability: A web tool is freely available for academic or commercial use at http://bioinfo.wistar.upenn.edu/siRNA/siRNA.htm. Source code is available upon request.

Supplementary information: See http://bioinfo.wistar.upenn.edu/siRNA/siRNA.htm for additional documentation and help pages.

Contact: rux{at}wistar.upenn.edu

* To whom correspondence should be addressed.


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