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Bioinformatics Advance Access originally published online on December 14, 2004
Bioinformatics 2005 21(8):1701-1702; doi:10.1093/bioinformatics/bti219
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© The Author 2004. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

MultiPLX: automatic grouping and evaluation of PCR primers

Lauris Kaplinski 1,2, Reidar Andreson 1, Tarmo Puurand 1 and Maido Remm 1,*

1Department of Bioinformatics, University of Tartu Tartu, Estonia
2Estonian Biocentre Riia str. 23, Tartu 51010, Estonia

*To whom correspondence should be addressed.

Summary: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer–primer interactions, primer–product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs.

Availability: Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users.

Contact: maido.remm{at}ut.ee


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 Nucleic Acids ResHome page
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[Abstract] [Full Text] [PDF]


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