Skip Navigation


Bioinformatics Advance Access originally published online on July 24, 2008
Bioinformatics 2008 24(18):2103-2104; doi:10.1093/bioinformatics/btn385
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (Print PDF) Freely available
Right arrow All Versions of this Article:
24/18/2103    most recent
btn385v1
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nelson, W. D.
Right arrow Articles by Lynn, B. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nelson, W. D.
Right arrow Articles by Lynn, B. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

An integrated approach for automating validation of extracted ion chromatographic peaks

William D. Nelson 1,*, Kert Viele 2 and Bert C. Lynn 3

1Department of Biology, 2Department of Statistics and 3Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA

*To whom correspondence should be addressed.


  Abstract

Summary: Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.

Contact: wnels2{at}uky.edu

Source and Supplementary Information: http://www.chem.uky.edu/research/lynn/Nelson.pdf

Associate Editor: David Rocke

Received on March 21, 2008; revised on July 18, 2008; accepted on July 22, 2008

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.