Skip Navigation


Bioinformatics Advance Access originally published online on March 14, 2008
Bioinformatics 2008 24(9):1214-1216; doi:10.1093/bioinformatics/btn090
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (Print PDF) Freely available
Right arrowOA All Versions of this Article:
24/9/1214    most recent
btn090v1
Right arrow Comments: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Comments are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Google Scholar
Right arrow Articles by Campbell, M. P.
Right arrow Articles by Rudd, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campbell, M. P.
Right arrow Articles by Rudd, P. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

GlycoBase and autoGU: tools for HPLC-based glycan analysis

Matthew P. Campbell *,{dagger}, Louise Royle {ddagger}, Catherine M. Radcliffe {dagger}, Raymond A. Dwek and Pauline M. Rudd {dagger}

Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK

*To whom correspondence should be addressed.


  Abstract

Summary: The development of robust high-performance liquid chromatography (HPLC) technologies continues to improve the detailed analysis and sequencing of glycan structures released from glycoproteins. Here, we present a database (GlycoBase) and analytical tool (autoGU) to assist the interpretation and assignment of HPLC-glycan profiles. GlycoBase is a relational database which contains the HPLC elution positions for over 350 2-AB labelled N-glycan structures together with predicted products of exoglycosidase digestions. AutoGU assigns provisional structures to each integrated HPLC peak and, when used in combination with exoglycosidase digestions, progressively assigns each structure automatically based on the footprint data. These tools are potentially very promising and facilitate basic research as well as the quantitative high-throughput analysis of low concentrations of glycans released from glycoproteins.

Availability: http://glycobase.ucd.ie

Contact: matthew.campbell{at}nibrt.ie

Associate Editor: Limsoon Wong

{dagger}Present address: Dublin-Oxford Glycobiology Laboratory, National Institute for Bioprocessing Research and Training, Conway Institute, University College Dublin, Dublin, Ireland.

{ddagger}Present address: Ludger Ltd, Culham Science Centre, Abingdon, Oxfordshire OX14 3EB., UK.

Received on February 8, 2008; revised on February 8, 2008; accepted on March 4, 2008

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 GlycobiologyHome page
R. Ranzinger, M. Frank, C.-W. von der Lieth, and S. Herget
Glycome-DB.org: A portal for querying across the digital world of carbohydrate sequences
Glycobiology, December 1, 2009; 19(12): 1563 - 1567.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.