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© 1987 IRL Press Ltd.

Hyperbolic regression analysis for kinetics, electrophoresis, ELISA, RIA, Bradford, Lowry, and other applications

Gary M. Studnicka

International Genetic Engineering, Inc. 1545 Seventeenth Street, Santa Monica, CA 90404, USA

Hyperbolic regression analysis is an effective method for fitting experimental data points obtained from a variety of experiments in molecular biology, including enzyme kinetics, agarose gel electrophoresis of DNA fragments, SDS—polyacrylamide gel electrophoresis of proteins, enzyme–linked immunosorbent assays (ELISA), radioimmunoassays (RIA), Bradford protein quantitation assays, Lowry protein assays, and other applications. Hyperbolic regression yields excellent fitted curves without the biases that are introduced by carrying out linear regression on double reciprocal coordinates, and it produces one simple equation, encompassing all the data points, that can be used easily in a pocket calculator to estimate the values of unknown samples from the known standards.

Received on February 9, 1986; accepted on April 11, 1986

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