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Bioinformatics Advance Access published online on April 8, 2004

Bioinformatics, doi:10.1093/bioinformatics/bth226
Bioinformatics © Oxford University Press 2004; all rights reserved
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Received December 23, 2003
Revised January 28, 2004
Accepted January 28, 2004

Applications note

Bellerophon: a program to detect chimeric sequences in multiple sequence alignments

Thomas Huber 1*, Geoffrey Faulkner 1, Philip Hugenholtz 2

1 ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia
2 Department of Environmental Science, Policy and Management, 151 Hilgard Hall, University of California Berkeley, Berkeley, CA 94720-3110, USA

* To whom correspondence should be addressed. E-mail: huber{at}maths.uq.edu.au.


   Abstract

Summary: Bellerophon is a program for detecting chimeric sequences in multiple sequence datasets by an adaption of partial treeing analysis. Bellerophon was specifically developed to detect 16S rRNA gene chimeras in PCR-clone libraries of environmental samples but can be applied to other nucleotide sequence alignments.

Availability: Bellerophon is available as an interactive web server at http://foo.maths.uq.edu.au/~huber/bellerophon.pl.


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Appl. Environ. Microbiol.Home page
V. Corby-Harris, A. C. Pontaroli, L. J. Shimkets, J. L. Bennetzen, K. E. Habel, and D. E. L. Promislow
Geographical Distribution and Diversity of Bacteria Associated with Natural Populations of Drosophila melanogaster
Appl. Envir. Microbiol., June 1, 2007; 73(11): 3470 - 3479.
[Abstract] [Full Text] [PDF]


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Proc R Soc BHome page
M. Pernice, S. Wetzel, O. Gros, R. Boucher-Rodoni, and N. Dubilier
Enigmatic dual symbiosis in the excretory organ of Nautilus macromphalus (Cephalopoda: Nautiloidea)
Proc R Soc B, May 7, 2007; 274(1614): 1143 - 1152.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
J. T. Lennon
Diversity and Metabolism of Marine Bacteria Cultivated on Dissolved DNA
Appl. Envir. Microbiol., May 1, 2007; 73(9): 2799 - 2805.
[Abstract] [Full Text] [PDF]


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Infect. Immun.Home page
C. R. Cox and M. S. Gilmore
Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis
Infect. Immun., April 1, 2007; 75(4): 1565 - 1576.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. Kimura, J.-I. Ishibashi, H. Masuda, K. Kato, and S. Hanada
Selective Phylogenetic Analysis Targeting 16S rRNA Genes of Hyperthermophilic Archaea in the Deep-Subsurface Hot Biosphere
Appl. Envir. Microbiol., April 1, 2007; 73(7): 2110 - 2117.
[Abstract] [Full Text] [PDF]


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Soil Sci.Home page
K. J. Martin
Introduction to Molecular Analysis of Ectomycorrhizal Communities
Soil Sci. Soc. Am. J., March 12, 2007; 71(2): 601 - 610.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
Z. Gao, C.-h. Tseng, Z. Pei, and M. J. Blaser
Molecular analysis of human forearm superficial skin bacterial biota
PNAS, February 20, 2007; 104(8): 2927 - 2932.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
E. L. Brodie, T. Z. DeSantis, J. P. M. Parker, I. X. Zubietta, Y. M. Piceno, and G. L. Andersen
Urban aerosols harbor diverse and dynamic bacterial populations
PNAS, January 2, 2007; 104(1): 299 - 304.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
G. W. Weidler, M. Dornmayr-Pfaffenhuemer, F. W. Gerbl, W. Heinen, and H. Stan-Lotter
Communities of Archaea and Bacteria in a Subsurface Radioactive Thermal Spring in the Austrian Central Alps, and Evidence of Ammonia-Oxidizing Crenarchaeota
Appl. Envir. Microbiol., January 1, 2007; 73(1): 259 - 270.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
S. Nakagawa, F. Inagaki, Y. Suzuki, B. O. Steinsbu, M. A. Lever, K. Takai, B. Engelen, Y. Sako, C. G. Wheat, K. Horikoshi, et al.
Microbial Community in Black Rust Exposed to Hot Ridge Flank Crustal Fluids
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6789 - 6799.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Hongoh, P. Deevong, S. Hattori, T. Inoue, S. Noda, N. Noparatnaraporn, T. Kudo, and M. Ohkuma
Phylogenetic Diversity, Localization, and Cell Morphologies of Members of the Candidate Phylum TG3 and a Subphylum in the Phylum Fibrobacteres, Recently Discovered Bacterial Groups Dominant in Termite Guts
Appl. Envir. Microbiol., October 1, 2006; 72(10): 6780 - 6788.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
K. E. Ashelford, N. A. Chuzhanova, J. C. Fry, A. J. Jones, and A. J. Weightman
New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras
Appl. Envir. Microbiol., September 1, 2006; 72(9): 5734 - 5741.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
W. J. Brazelton, M. O. Schrenk, D. S. Kelley, and J. A. Baross
Methane- and Sulfur-Metabolizing Microbial Communities Dominate the Lost City Hydrothermal Field Ecosystem
Appl. Envir. Microbiol., September 1, 2006; 72(9): 6257 - 6270.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
E. L. Brodie, T. Z. DeSantis, D. C. Joyner, S. M. Baek, J. T. Larsen, G. L. Andersen, T. C. Hazen, P. M. Richardson, D. J. Herman, T. K. Tokunaga, et al.
Application of a High-Density Oligonucleotide Microarray Approach To Study Bacterial Population Dynamics during Uranium Reduction and Reoxidation
Appl. Envir. Microbiol., September 1, 2006; 72(9): 6288 - 6298.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
M. Scheuermayer, T. A. M. Gulder, G. Bringmann, and U. Hentschel
Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum 'Verrucomicrobia', isolated from a sponge (Porifera).
Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2119 - 2124.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
J. L. Macalady, E. H. Lyon, B. Koffman, L. K. Albertson, K. Meyer, S. Galdenzi, and S. Mariani
Dominant Microbial Populations in Limestone-Corroding Stream Biofilms, Frasassi Cave System, Italy
Appl. Envir. Microbiol., August 1, 2006; 72(8): 5596 - 5609.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
S. Lin, H. Zhang, Y. Hou, L. Miranda, and D. Bhattacharya
Development of a Dinoflagellate-Oriented PCR Primer Set Leads to Detection of Picoplanktonic Dinoflagellates from Long Island Sound
Appl. Envir. Microbiol., August 1, 2006; 72(8): 5626 - 5630.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
T. Z. DeSantis, P. Hugenholtz, N. Larsen, M. Rojas, E. L. Brodie, K. Keller, T. Huber, D. Dalevi, P. Hu, and G. L. Andersen
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB.
Appl. Envir. Microbiol., July 1, 2006; 72(7): 5069 - 5072.
[Abstract] [Full Text] [PDF]


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Nucleic Acids ResHome page
T. Z. DeSantis Jr, P. Hugenholtz, K. Keller, E. L. Brodie, N. Larsen, Y. M. Piceno, R. Phan, and G. L. Andersen
NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes.
Nucleic Acids Res., July 1, 2006; 34(Web Server issue): W394 - W399.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. Sanguin, B. Remenant, A. Dechesne, J. Thioulouse, T. M. Vogel, X. Nesme, Y. Moenne-Loccoz, and G. L. Grundmann
Potential of a 16S rRNA-Based Taxonomic Microarray for Analyzing the Rhizosphere Effects of Maize on Agrobacterium spp. and Bacterial Communities.
Appl. Envir. Microbiol., June 1, 2006; 72(6): 4302 - 4312.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
R. E. Ley, J. K. Harris, J. Wilcox, J. R. Spear, S. R. Miller, B. M. Bebout, J. A. Maresca, D. A. Bryant, M. L. Sogin, and N. R. Pace
Unexpected diversity and complexity of the guerrero negro hypersaline microbial mat.
Appl. Envir. Microbiol., May 1, 2006; 72(5): 3685 - 3695.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
V. Souza, L. Espinosa-Asuar, A. E. Escalante, L. E. Eguiarte, J. Farmer, L. Forney, L. Lloret, J. M. Rodriguez-Martinez, X. Soberon, R. Dirzo, et al.
From the Cover: An endangered oasis of aquatic microbial biodiversity in the Chihuahuan desert
PNAS, April 25, 2006; 103(17): 6565 - 6570.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
J. Kirkpatrick, B. Oakley, C. Fuchsman, S. Srinivasan, J. T. Staley, and J. W. Murray
Diversity and Distribution of Planctomycetes and Related Bacteria in the Suboxic Zone of the Black Sea
Appl. Envir. Microbiol., April 1, 2006; 72(4): 3079 - 3083.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
S. N. Dedysh, T. A. Pankratov, S. E. Belova, I. S. Kulichevskaya, and W. Liesack
Phylogenetic analysis and in situ identification of bacteria community composition in an acidic sphagnum peat bog.
Appl. Envir. Microbiol., March 1, 2006; 72(3): 2110 - 2117.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Taprab, T. Johjima, Y. Maeda, S. Moriya, S. Trakulnaleamsai, N. Noparatnaraporn, M. Ohkuma, and T. Kudo
Symbiotic Fungi Produce Laccases Potentially Involved in Phenol Degradation in Fungus Combs of Fungus-Growing Termites in Thailand
Appl. Envir. Microbiol., December 1, 2005; 71(12): 7696 - 7704.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
K. E. Ashelford, N. A. Chuzhanova, J. C. Fry, A. J. Jones, and A. J. Weightman
At Least 1 in 20 16S rRNA Sequence Records Currently Held in Public Repositories Is Estimated To Contain Substantial Anomalies
Appl. Envir. Microbiol., December 1, 2005; 71(12): 7724 - 7736.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
T. E. Freitag, L. Chang, C. D. Clegg, and J. I. Prosser
Influence of Inorganic Nitrogen Management Regime on the Diversity of Nitrite-Oxidizing Bacteria in Agricultural Grassland Soils
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8323 - 8334.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
D. P. Moser, T. M. Gihring, F. J. Brockman, J. K. Fredrickson, D. L. Balkwill, M. E. Dollhopf, B. S. Lollar, L. M. Pratt, E. Boice, G. Southam, et al.
Desulfotomaculum and Methanobacterium spp. Dominate a 4- to 5-Kilometer-Deep Fault
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8773 - 8783.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
A. K. Dunn and E. V. Stabb
Culture-Independent Characterization of the Microbiota of the Ant Lion Myrmeleon mobilis (Neuroptera: Myrmeleontidae)
Appl. Envir. Microbiol., December 1, 2005; 71(12): 8784 - 8794.
[Abstract] [Full Text] [PDF]


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Int. J. Syst. Evol. Microbiol.Home page
S. von der Heyden and T. Cavalier-Smith
Culturing and environmental DNA sequencing uncover hidden kinetoplastid biodiversity and a major marine clade within ancestrally freshwater Neobodo designis
Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2605 - 2621.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
Y. Hongoh, P. Deevong, T. Inoue, S. Moriya, S. Trakulnaleamsai, M. Ohkuma, C. Vongkaluang, N. Noparatnaraporn, and T. Kudo
Intra- and Interspecific Comparisons of Bacterial Diversity and Community Structure Support Coevolution of Gut Microbiota and Termite Host
Appl. Envir. Microbiol., November 1, 2005; 71(11): 6590 - 6599.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
K. B. Sorensen, D. E. Canfield, A. P. Teske, and A. Oren
Community Composition of a Hypersaline Endoevaporitic Microbial Mat
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7352 - 7365.
[Abstract] [Full Text] [PDF]


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Proc R Soc BHome page
J. Slapeta, D. Moreira, and P. Lopez-Garcia
The extent of protist diversity: insights from molecular ecology of freshwater eukaryotes
Proc R Soc B, October 7, 2005; 272(1576): 2073 - 2081.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
H. M. Simon, C. E. Jahn, L. T. Bergerud, M. K. Sliwinski, P. J. Weimer, D. K. Willis, and R. M. Goodman
Cultivation of Mesophilic Soil Crenarchaeotes in Enrichment Cultures from Plant Roots
Appl. Envir. Microbiol., August 1, 2005; 71(8): 4751 - 4760.
[Abstract] [Full Text] [PDF]


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Appl. Environ. Microbiol.Home page
C. F. V. Hobel, V. T. Marteinsson, G. O. Hreggvidsson, and J. K. Kristjansson
Investigation of the Microbial Ecology of Intertidal Hot Springs by Using Diversity Analysis of 16S rRNA and Chitinase Genes
Appl. Envir. Microbiol., May 1, 2005; 71(5): 2771 - 2776.
[Abstract] [Full Text] [PDF]



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